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For holding of oocytes prior to maturation
20 ml medium in glass bottle
For holding of oocytes to postpone maturation
Oocyte Holding must be supplemented with 10% -20% serum
Medium should be preheated to 22 °C prior to use
The medium is HEPES buffered and does not require CO2 equilibration
Inorganic Salts, Essential and non-essential Amino Acids, Vitamins, Sodium Bicarbonate, Alanyl Glutamine, Glucose, Albumin, Phenol Red, Tween 80, HEPES and Gentamycin
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