Sperm DNA Fragmentation (SCD Method)

Description

The CARIAD Sperm DNA Fragmentation Kit employs the Sperm Chromatin Diffusion (SCD) method to assess sperm DNA integrity, crucial for male fertility. Sperm DNA fragmentation (SDF) indicates the presence of sperm with damaged DNA, affecting fertility and reproductive outcomes.

Description

This kit is based on the observation that sperm with fragmented DNA do not show the typical halo of dispersed DNA loops seen in sperm with intact DNA, after acid denaturation and removal of nuclear proteins. Additionally, the SCD test can be utilized to evaluate sperm DNA longevity, revealing latent DNA damage not immediately apparent during initial assessments.

Features

• Contain dithiothreitol lysis solution and Wright staining solution
• Easy identification: sperm with fragmented DNA does not show a dispersion halo or the halo is minimal
• 20 tests/kit, stable for 12 months at 2-8℃