The pT-100 is a compact and simple-to-use LED illumination system for transmitted light applications, including brightfield, darkfield, DIC, Dodt gradient contrast and phase contrast.
A true 100 W Halogen replacement.
Description
The system is available in four variants to bring the benefits of LEDs to modern laboratories. A broad white system provides a safer and convenient alternative to halogen by reducing harmful UV exposure to the eyes and is ideal for long hours spent at the microscope, while three narrow bandwidth options (525, 635 and 770 nm) optimise image quality through increased contrast.
The pT-100-WHT and pT-100-770 nm are also available as variants with a 3 mm liquid light guide attachment, which is ideal for electrophysiology applications.
Samples will appear identical even though irradiance could be up to five times greater.
In addition, the pT-100-WHT does not produce unwanted light in the UV which makes it safer and more comfortable, as risk of eye damage is significantly lower.
The pT-100-WHT and pT-100-770 nm are also available with a 3 mm liquid light guide as an alternative to using a direct attachment adaptor, offering two main benefits:
- Enhancing accuracy of sensitive electrophysiology experiments as remote positioning allows the Illumination Systems to be placed outside of a faraday cage, avoiding electrical interference.
- Added compatibility with microscopes which only feature a liquid light guide input, the collimating optics being an integral part of the microscope.
pT-100-WHT:
- Comfortable operation and reduced risk of eye damage as no unwanted UV
- Spectral performance matched to 100 W halogen, creating consistent images
- No variation in colour across irradiance range, removing the need to colour balance
- Replaces 100 W halogen lamp housing on most current and older microscopes – quick & easy exchange
- LLG version enhances accuracy for electrophysiology applications
pT-100-525:
- A phosphor-free illumination centred around 525 nm optimised for the spectral response of most scientific cameras
- Improved image contrast compared to white light source
pT-100-635:
- A phosphor-free solution for brightfield imaging with a peak of 635 nm providing deeper penetration in thicker samples
- Reduced phototoxicity of light-sensitive samples
pT-100-770:
- A specialised solution for IR contrast techniques such as DIC, Dodt contrast etc.
- Centred around 770 nm provides the optimal output for deeper sample penetration techniques requiring infrared light
- LLG version enhances accuracy of electrophysiology applications using thick tissue slices
Specifications
The system comprises a pT-100 LED Light Source, control pod, and power supply.
Power requirements
100-240 V a.c. 50/60 Hz
Power consumption
1 W (standby)
20 W (full irradiance)
Manual:
Manual control for instant on/off and irradiance control in 1% steps from 0 – 100%
Remote:
Via single TTL for on/off control using a BNC connection on the control pod. Triggering speed <150 µs
Direct fit to a microscope
Specify a Microscope Adaptor from CoolLED’s range of transmitted ports
Testimonials
For temperature sensitive applications such as ours, we strongly recommend changing conventional halogen-driven transmitted light sources for LED units such as the pT-100-WHT.
Dimitri Kalleas & Alex Laude, Newcastle University
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Set up was very easy and pT-100 Light Source was simple to operate. LEDs gave nice even illumination at 2% power. Higher powers were not necessary due to the brightness of the LED: 2% power with 5 ms or 10 ms exposure. There were no obvious signs of cell stress due to light toxicity over the duration of the 60 min or 180 min experiments I ran. Overall they are neat, simple to use and very efficient.
Tim Self, Head of School of Life Sciences imaging (SLIM), University of Nottingham, UK
Brochure
Brochure
