For sperm swim up
50 ml medium in glass bottle for semen washing
The medium is HEPES buffered, and does not need CO2 equilibration, however, the medium can be placed inside the incubator during the swim up procedure
Place tube at an angle to maximize surface to harvest more spermatozoa
The medium is ready-to-use and does not need any supplements
Medium should be preheated to 38,2°C prior to use