The Calibre Scientific PiezoXpert ensures gentle and effective penetration of the cell membrane, thereby improving the outcome of your cell manipulation experiments.
Overview
The Calibre Scientific PiezoXpert is a high-performance piezo-assisted micromanipulation system designed to enable precise cell penetration for microinjection and advanced micromanipulation applications. By delivering piezo impulses directly to the capillary without any loss of intensity, the system supports efficient and controlled perforation of the cell membrane, improving accuracy and consistency during delicate procedures.
Piezo-assisted micromanipulation enhances outcomes across a wide range of research applications, including the transfer of embryonic and induced pluripotent stem (iPS) cells into blastocysts and morulae, microinjection into mammalian oocytes and zygotes such as Piezo-ICSI, and enucleation and nuclear transfer techniques.
The Calibre Scientific PiezoXpert is fully compatible with Calibre Scientific micromanipulators, providing a seamless integrated solution for micromanipulation workflows. It can also be easily incorporated into existing non-Calibre Scientific micromanipulation systems, offering flexibility for laboratories with established equipment.
Overview: Piezo-ICSI vs. Conventional ICSI
- Significantly reduced deformation of the oocyte during ICSI-related penetration
- Involves a specialized Piezo-actuator to generate high-frequency, low-amplitude mechanical pulses
- Use of a blunt ICSI-microcapillary to ensure optimal mechanical force transfer
- Several studies have shown significantly higher oocyte survival rates
Applications
- Piezo-ICSI (Intracytoplasmic Sperm Injection) in mouse, equine, porcine etc.
- Transfer of embryonic or induced pluripotent stem cells into blastocysts or morulae
- Enucleation/nuclear transfer
- Blastomere biopsy from mouse embryos
- Biopsy of equine embryos for Preimplantation Genetic Testing
- RNA/DNA injection into fertilized oocytes of mammals
Features
- Fast and simple sample processing due to intuitive operation
- Reproducible parameter setting
- Program keys to save your experimental parameters
- Trigger impulses with an ergonomic foot control or rotary knobs right on the device
- Optical feedback of triggered impulses
- Additional "Clean" function for simple removal of contamination on the outside of the capillary
- Adapters are available for the PiezoXpert to be fitted onto most Micromanipulators
Brochure
Brochure
