Small Ruminant - Vitrification kit
Vitrification Kit
3 Vials for Embryo Vitrification
For vitrification of in vitro and in vivo embryos
Description
3 vials each containing 2 ml medium in glass bottles, Prod. No. 6.20.006
Obtain higher embryo survival and pregnancy rates with vitrification than after slow freeze Particularly in vitro and biopsied embryos benefit from this cryopreservation method
Media should not be pre-heated to more than 30°C prior to use – leave lid on in order to avoid evaporation
Key Ingredients
Sucrose, Ficoll, Dimethyl Sulphoxide (DMSO) Ethylene Glycol, Glucose, Sodium Pyruvate, Albumin, Inorganic Salts. The cryoprotective ingredients work by dehydrating embryos combined with an influx of high concentrations of cryoprotectants by increasing osmolality stepwise from 290 mOsm/kg to 865 mOsm/kg and the reverse procedure for warming (rehydration of embryo and removal of cryoprotectants) Above steps are essential for rapid cooling into LN2, which takes place from 30 °C to −196 °C in less than one second See the detailed protocol on Vitrification and Warming of embryos
Quality Control Tests and Manufacturing
- Sterility tested (Fungal and Bacterial)
- Osmolality tested and pH tested
- Endotoxin tested
- Manufacturer is ISO9001 and ISO13485 certified
- Controlled environment and to human standards
- Bovine Embryo Assay (BEA)
- Batch analysis certificate available
Storage
- Store in original unopened container refrigerated 3-9 °C and protected from light
- Do not expose to temperatures higher than 39°C
- Do not freeze product it may precipitate < 2°C
- The medium must be used within 10 days after opening, providing it has been
- handled aseptically and is kept refrigerated after opening
- Discard unused medium if it has been warmed