Papanicolaou Staining Solution for Spermatozoan Morpology
CARIAD Papanicolaou Staining Solution (PAP) stain semen samples for sperm morphology.
• WHO recommended method, excellent staining effect
• Hematoxylin, Eosin Azure 50 and Orange Green stains
Features
Distinct morphological features after staining summary. Nucleus have a positive dye reaction, cytoplasm does not.
Hematoxylin : Natural dye hematoxylin is the nuclear stain which will stain nuclei blue. It has affinity for chromatin, attaching to sulphate groups on the D.N.A. molecule.
Eosin Azure : This is the second counterstain which is a polychrome mixture of eosin Y, light green SF and Bismarck brown.
Orange Green 6 : This is the first acidic counterstain (cytoplasmic stain) which stains matured and keratinized cells. The target structures are stained orange in different intensities.
Sizes
• 500 mL×5
Shelf Life
Stable for 12 months at room temperature and is valid for 30 days after opening