For the in vitro maturation of oocytes outside the incubator
20 ml HEPES IVM medium in glass bottle
The medium is HEPES buffered and does not require CO2equilibration, but it can also be used in a CO2Incubator (recommended without vial lid, if oocytes arrive to the laboratory before 20 hours of after IVM)
The medium isready-to-useand does not need any supplements
Medium should be preheated to 38.8°C prior to use
Key Ingredients
HEPES, FSH and LH, Epidermal Growth Factor (EGF), Midkine (MK), L-Carnitine, Sodium Pyruvate, Albumin, Inorganic Salts, Essentiel and Non-Essentiel Amino Acids, Vitamins, Sodium Bicarbonate, L-Glutamine, Glucose, Phenol Red, and Gentamycin
Quality Control Tests and Manufacturing
Sterility tested (Fungal and Bacterial)
Osmolality tested and pH tested
Endotoxin tested
Manufacturer is ISO9001 and ISO13485 certified
Controlled environment and to human standards
Bovine Embryo Assay (BEA)
Batch analysis certificate available
Storage:
Store in original unopened container refrigerated 3-9 °C and protected from light
Do not expose to temperatures higher than 39°C
Do not freeze product it may precipitate < 2°C
The medium must be used within 10 days after opening, providing it has been
handled aseptically and is kept refrigerated after opening
